rabbit polyclonal anti p akt 473 (Proteintech)
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Rabbit Polyclonal Anti P Akt 473, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 527 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+polyclonal+anti+p+akt+473/Phospho-AKT1+(Ser473)+Antibody/pmc11727224-91-35-42
Average 96 stars, based on 527 article reviews
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Membrane:Article Title: TREM2 affects DAM-like cell transformation in the acute phase of TBI in mice by regulating microglial glycolysis. Article Snippet: Equal amounts of protein (20 μg) from each sample were added to 4× Laemmli sample buffer and subjected to sodium dodecyl sulfate‒polyacrylamide gel electrophoresis (SDS‒PAGE) (Invitrogen), followed by transfer onto a polyvinylidene fluoride (PVDF) membrane (Millipore, Boston, MA, USA). .. The membrane was washed with TBST buffer (Tris-buffered saline containing 0.1% Tween 20), blocked with 5% nonfat milk for 1 h at room temperature, and subsequently incubated overnight at 4 °C with primary antibodies, including Article Title: TREM2 affects DAM-like cell transformation in the acute phase of TBI in mice by regulating microglial glycolysis Article Snippet: Equal amounts of protein (20 μg) from each sample were added to 4× Laemmli sample buffer and subjected to sodium dodecyl sulfate‒polyacrylamide gel electrophoresis (SDS‒PAGE) (Invitrogen), followed by transfer onto a polyvinylidene fluoride (PVDF) membrane (Millipore, Boston, MA, USA). .. The membrane was washed with TBST buffer (Tris-buffered saline containing 0.1% Tween 20), blocked with 5% non-fat milk for 1 h at room temperature, and subsequently incubated overnight at 4 °C with primary antibodies, including Article Title: TREM2 affects DAM-like cell transformation in the acute phase of TBI in mice by regulating microglial glycolysis Article Snippet: Equal amounts of protein (20 μg) from each sample were added to 4× Laemmli sample buffer and subjected to sodium dodecyl sulfate‒polyacrylamide gel electrophoresis (SDS‒PAGE) (Invitrogen), followed by transfer onto a polyvinylidene uoride (PVDF) membrane (Millipore, Boston, MA, USA). .. The membrane was washed with TBST buffer (Tris-buffered saline containing 0.1% Tween 20), blocked with 5% nonfat milk for 1 h at room temperature, and subsequently incubated overnight at 4°C with primary antibodies, including Saline:Article Title: TREM2 affects DAM-like cell transformation in the acute phase of TBI in mice by regulating microglial glycolysis. Article Snippet: Equal amounts of protein (20 μg) from each sample were added to 4× Laemmli sample buffer and subjected to sodium dodecyl sulfate‒polyacrylamide gel electrophoresis (SDS‒PAGE) (Invitrogen), followed by transfer onto a polyvinylidene fluoride (PVDF) membrane (Millipore, Boston, MA, USA). .. The membrane was washed with TBST buffer (Tris-buffered saline containing 0.1% Tween 20), blocked with 5% nonfat milk for 1 h at room temperature, and subsequently incubated overnight at 4 °C with primary antibodies, including Article Title: TREM2 affects DAM-like cell transformation in the acute phase of TBI in mice by regulating microglial glycolysis Article Snippet: Equal amounts of protein (20 μg) from each sample were added to 4× Laemmli sample buffer and subjected to sodium dodecyl sulfate‒polyacrylamide gel electrophoresis (SDS‒PAGE) (Invitrogen), followed by transfer onto a polyvinylidene fluoride (PVDF) membrane (Millipore, Boston, MA, USA). .. The membrane was washed with TBST buffer (Tris-buffered saline containing 0.1% Tween 20), blocked with 5% non-fat milk for 1 h at room temperature, and subsequently incubated overnight at 4 °C with primary antibodies, including Article Title: TREM2 affects DAM-like cell transformation in the acute phase of TBI in mice by regulating microglial glycolysis Article Snippet: Equal amounts of protein (20 μg) from each sample were added to 4× Laemmli sample buffer and subjected to sodium dodecyl sulfate‒polyacrylamide gel electrophoresis (SDS‒PAGE) (Invitrogen), followed by transfer onto a polyvinylidene uoride (PVDF) membrane (Millipore, Boston, MA, USA). .. The membrane was washed with TBST buffer (Tris-buffered saline containing 0.1% Tween 20), blocked with 5% nonfat milk for 1 h at room temperature, and subsequently incubated overnight at 4°C with primary antibodies, including Incubation:Article Title: TREM2 affects DAM-like cell transformation in the acute phase of TBI in mice by regulating microglial glycolysis. Article Snippet: Equal amounts of protein (20 μg) from each sample were added to 4× Laemmli sample buffer and subjected to sodium dodecyl sulfate‒polyacrylamide gel electrophoresis (SDS‒PAGE) (Invitrogen), followed by transfer onto a polyvinylidene fluoride (PVDF) membrane (Millipore, Boston, MA, USA). .. The membrane was washed with TBST buffer (Tris-buffered saline containing 0.1% Tween 20), blocked with 5% nonfat milk for 1 h at room temperature, and subsequently incubated overnight at 4 °C with primary antibodies, including Article Title: TREM2 affects DAM-like cell transformation in the acute phase of TBI in mice by regulating microglial glycolysis Article Snippet: Equal amounts of protein (20 μg) from each sample were added to 4× Laemmli sample buffer and subjected to sodium dodecyl sulfate‒polyacrylamide gel electrophoresis (SDS‒PAGE) (Invitrogen), followed by transfer onto a polyvinylidene fluoride (PVDF) membrane (Millipore, Boston, MA, USA). .. The membrane was washed with TBST buffer (Tris-buffered saline containing 0.1% Tween 20), blocked with 5% non-fat milk for 1 h at room temperature, and subsequently incubated overnight at 4 °C with primary antibodies, including Article Title: TREM2 affects DAM-like cell transformation in the acute phase of TBI in mice by regulating microglial glycolysis Article Snippet: Equal amounts of protein (20 μg) from each sample were added to 4× Laemmli sample buffer and subjected to sodium dodecyl sulfate‒polyacrylamide gel electrophoresis (SDS‒PAGE) (Invitrogen), followed by transfer onto a polyvinylidene uoride (PVDF) membrane (Millipore, Boston, MA, USA). .. The membrane was washed with TBST buffer (Tris-buffered saline containing 0.1% Tween 20), blocked with 5% nonfat milk for 1 h at room temperature, and subsequently incubated overnight at 4°C with primary antibodies, including |
